Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody, PBS Only

LAG-3/CD223 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241294E11

Cat no : 98167-1-PBS

Synonyms

CD223, LAG-3, Ly66, Lag3, LAG 3

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 98167-1-RR (same clone as 98167-1-PBS)

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone: 241294E11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98167-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98167-1-RR (same clone as 98167-1-PBS)

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone: 241294E11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone:241294E11) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98167-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98167-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98167-1-RR against Mouse LAG-3/CD223 were performed. The affinity constant is 0.636 nM.

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Product Information

The immunogen of 98167-1-PBS is LAG-3/CD223 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: lymphocyte-activation gene 3
• Calculated Molecular Weight: 57kDa
• GenBank Accession Number: NM_008479.2
• Gene Symbol: CD223
• Gene ID (NCBI): 16768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).