Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22221-1-AP

Synonyms

KRT14, keratin 14, Cytokeratin-14, Cytokeratin,Keratin, CK-14

---

Validation Data Gallery

• 

  WB analysis using 22221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 22221-1-AP

  IP result of anti-Cytokeratin 14 (IP:22221-1-AP, 4ug; Detection:22221-1-AP 1:1000) with mouse skin tissue lysate 3200ug.

• 

  IHC staining of human skin using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human bowen disease using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cervical cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse skin using 22221-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 14 antibody (22221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skin using 22221-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 14 antibody (22221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse skin tissue, rat skin tissue
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: human skin tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

22221-1-AP targets Cytokeratin 14 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 14 fusion protein Ag17559
• Full Name: keratin 14
• Calculated Molecular Weight: 472 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC002690
• Gene Symbol: Cytokeratin 14
• Gene ID (NCBI): 3861
• RRID: AB_2879036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. This antibody is specifically against KRT14.

Publications

Species	Application	Title
human, hamster	WB	Cancer Gene Ther; Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model.; Authors - S Vijayalingam