KRAS-2A-specific Monoclonal antibody

KRAS-2A-specific Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C8F5

Cat no : 67266-1-Ig

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS-2A-specific, KRAS2B, KRAS2B speicifc, Krasa, Krasb, NS3, RASK2

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Validation Data Gallery

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  WB analysis using 67266-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67266-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IF Staining of HeLa using 67266-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, SH-SY5Y cells, PC-12 cells, Neuro-2a cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67266-1-Ig targets KRAS-2A-specific in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_033360
• Gene Symbol: KRAS
• Gene ID (NCBI): 3845
• RRID: AB_2882537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.