KRAS-4A-specific Monoclonal antibody

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  WB analysis using 67266-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67266-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HeLa using 67266-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

KRAS-4A-specific Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67266-1-Ig

Clone No.3C8F5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

KRAS-4A, KRAS, pan RAS, EC:3.6.5.2, c-K-ras

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, SH-SY5Y cells, PC-12 cells, Neuro-2a cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67266-1-Ig targets KRAS-4A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_033360
• Gene Symbol: KRAS
• Gene ID (NCBI): 3845
• RRID: AB_2882537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01116
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 4A and 4B. This antibody is specific for KRAS-4A, which is also named as KRAS-2A.