Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, SH-SY5Y cells, PC-12 cells, Neuro-2a cells |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67266-1-Ig targets KRAS-4A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide Predict reactive species |
Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | NM_033360 |
Gene Symbol | KRAS |
Gene ID (NCBI) | 3845 |
RRID | AB_2882537 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P01116 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 4A and 4B. This antibody is specific for KRAS-4A, which is also named as KRAS-2A.
Protocols
Product Specific Protocols | |
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WB protocol for KRAS-4A-specific antibody 67266-1-Ig | Download protocol |
IF protocol for KRAS-4A-specific antibody 67266-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |