KLF13 Polyclonal antibody

• Featured Product
• KD/KO Validated

KLF13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 18352-1-AP

Synonyms

BTE binding protein 3, BTEB3, FKLF2, KLF13, Krueppel like factor 13, Kruppel like factor 13, NSLP1, RFLAT 1, RFLAT1, Transcription factor BTEB3, Transcription factor NSLP1

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Validation Data Gallery

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  WB analysis using 18352-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18352-1-AP (KLF13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 18352-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18352-1-AP (KLF13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18352-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18352-1-AP (KLF13 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HT-29 cells, rat liver tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

18352-1-AP targets KLF13 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLF13 fusion protein Ag13200
• Full Name: Kruppel-like factor 13
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC012741
• Gene Symbol: KLF13
• Gene ID (NCBI): 51621
• RRID: AB_2132399
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kruppel-like factor 13(KLF13) is a member of transcription actors, which contains 3 zinc finger DNA-binding domains. Theses transcription factors bind to CG-rich sequences and related GT and CACCC boxes. KLF13 involved in activation of transcription of variety of genes in the cells of erythroid lineage, such as GATA1, glycophorin B. It also binds to the A and A/B RANTES promoter binding sites, the same as NFKB, and then transactivates the RANTES gene. The calculated molecular weight of KLF13 is 31 kDa, but the modified KLF13 protein is about 37-45 kDa.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB,IF	J Hepatocell Carcinoma; Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma.; Authors - Xingming Xie
mouse	WB,IF	J Biol Chem; Krüppel-Like Factor 13 is a major mediator of Glucocorticoid Receptor-Signaling in Cardiomyocytes and protects these Cells from DNA Damage and Death.; Authors - Diana Cruz-Topete
rat	WB	Placenta; TET3-mediated novel regulatory mechanism affecting trophoblast invasion and migration: Implications for preeclampsia development; Authors - Cong Wang
mouse	WB	Hum Cell; Downregulation of miR-96 suppresses the profibrogenic functions of cardiac fibroblasts induced by angiotensin II and attenuates atrial fibrosis by upregulating KLF13.; Authors - Lijie Su ; KD Validated
human	WB	Cell Biosci; KLF13 suppresses the proliferation and growth of colorectal cancer cells through transcriptionally inhibiting HMGCS1-mediated cholesterol biosynthesis.; Authors - Weilong Yao ; KD Validated