|Positive WB detected in||HT-29 cells, rat liver tissue|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
18352-1-AP targets KLF13 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLF13 fusion protein Ag13200|
|Full Name||Kruppel-like factor 13|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||37-45 kDa|
|GenBank accession number||BC012741|
|Gene ID (NCBI)||51621|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Kruppel-like factor 13(KLF13) is a member of transcription actors, which contains 3 zinc finger DNA-binding domains. Theses transcription factors bind to CG-rich sequences and related GT and CACCC boxes. KLF13 involved in activation of transcription of variety of genes in the cells of erythroid lineage, such as GATA1, glycophorin B. It also binds to the A and A/B RANTES promoter binding sites, the same as NFKB, and then transactivates the RANTES gene. The calculated molecular weight of KLF13 is 31 kDa, but the modified KLF13 protein is about 37-45 kDa.
Divergent transcriptional regulation of astrocyte reactivity across disorders.
J Hepatocell Carcinoma
Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma.
J Biol Chem
Krüppel-Like Factor 13 is a major mediator of Glucocorticoid Receptor-Signaling in Cardiomyocytes and protects these Cells from DNA Damage and Death.
Downregulation of miR-96 suppresses the profibrogenic functions of cardiac fibroblasts induced by angiotensin II and attenuates atrial fibrosis by upregulating KLF13.
KLF13 suppresses the proliferation and growth of colorectal cancer cells through transcriptionally inhibiting HMGCS1-mediated cholesterol biosynthesis.
Onco Targets Ther
Downregulation of KLF13 through DNMT1-mediated hypermethylation promotes glioma cell proliferation and invasion.