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  • KD/KO Validated

KLF13 Polyclonal antibody

KLF13 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18352-1-AP

Synonyms

BTE binding protein 3, BTEB3, FKLF2, KLF13, Krueppel like factor 13, Kruppel like factor 13, NSLP1, RFLAT 1, RFLAT1, Transcription factor BTEB3, Transcription factor NSLP1



Tested Applications

Positive WB detected inHT-29 cells, rat liver tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18352-1-AP targets KLF13 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF13 fusion protein Ag13200
Full Name Kruppel-like factor 13
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 37-45 kDa
GenBank Accession NumberBC012741
Gene Symbol KLF13
Gene ID (NCBI) 51621
RRIDAB_2132399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kruppel-like factor 13(KLF13) is a member of transcription actors, which contains 3 zinc finger DNA-binding domains. Theses transcription factors bind to CG-rich sequences and related GT and CACCC boxes. KLF13 involved in activation of transcription of variety of genes in the cells of erythroid lineage, such as GATA1, glycophorin B. It also binds to the A and A/B RANTES promoter binding sites, the same as NFKB, and then transactivates the RANTES gene. The calculated molecular weight of KLF13 is 31 kDa, but the modified KLF13 protein is about 37-45 kDa.

Protocols

Product Specific Protocols
WB protocol for KLF13 antibody 18352-1-APDownload protocol
IHC protocol for KLF13 antibody 18352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanWB,IF

J Hepatocell Carcinoma

Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma.

Authors - Xingming Xie
mouseWB,IF

J Biol Chem

Krüppel-Like Factor 13 is a major mediator of Glucocorticoid Receptor-Signaling in Cardiomyocytes and protects these Cells from DNA Damage and Death.

Authors - Diana Cruz-Topete
mouseWB

Hum Cell

Downregulation of miR-96 suppresses the profibrogenic functions of cardiac fibroblasts induced by angiotensin II and attenuates atrial fibrosis by upregulating KLF13.

Authors - Lijie Su
  • KD Validated
humanWB

Cell Biosci

KLF13 suppresses the proliferation and growth of colorectal cancer cells through transcriptionally inhibiting HMGCS1-mediated cholesterol biosynthesis.

Authors - Weilong Yao
  • KD Validated
humanWB

Onco Targets Ther

Downregulation of KLF13 through DNMT1-mediated hypermethylation promotes glioma cell proliferation and invasion.

Authors - Rile Wu