KIF3C Polyclonal antibody

KIF3C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 14333-1-AP

Synonyms

Kinesin-like protein KIF3C

---

Validation Data Gallery

• 

  WB analysis of Y79 using 14333-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14333-1-AP (KIF3C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14333-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14333-1-AP (KIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14333-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14333-1-AP (KIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14333-1-AP

  IP result of anti-KIF3C (IP:14333-1-AP, 4ug; Detection:14333-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human gliomas using 14333-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14333-1-AP (KIF3C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14333-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14333-1-AP (KIF3C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 14333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 14333-1-AP (KIF3C antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: Y79 cells, HeLa cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

14333-1-AP targets KIF3C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF3C fusion protein Ag5619
• Full Name: kinesin family member 3C
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC042486
• Gene Symbol: KIF3C
• Gene ID (NCBI): 3797
• RRID: AB_1288727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran
mouse	WB	Elife; Loss of Tsc1 in cerebellar Purkinje cells induces transcriptional and translation changes in FMRP target transcripts; Authors - Jasbir Singh Dalal
human	IHC	Aging (Albany NY); METTL3-mediated m6A modification of KIF3C-mRNA promotes prostate cancer progression and is negatively regulated by miR-320d.; Authors - Honggui Ma
human	WB	Biomed Res Int; KIF3C Promotes Proliferation, Migration, and Invasion of Glioma Cells by Activating the PI3K/AKT Pathway and Inducing EMT.; Authors - Yang Gao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Abigail (Verified Customer) (07-10-2024)

We used this antibody for WB of mouse brain cortex homogenate and got clear bands around 100 kDa after a 1:1000 overnight incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain cortex