|Positive WB detected in||Caco-2 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15911-1-AP targets KIF20A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KIF20A fusion protein Ag8700|
|Full Name||kinesin family member 20A|
|Calculated molecular weight||890 aa, 100 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC012999|
|Gene ID (NCBI)||10112|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.
Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions.
Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.
Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.
J Biochem Mol Toxicol
Bisphenol A triggers the malignancy of acute myeloid leukemia cells via regulation of IL-4 and IL-6.
KIF20A Predicts Poor Survival of Patients and Promotes Colorectal Cancer Tumor Progression through the JAK/STAT3 Signaling Pathway.
The mechanism of acentrosomal spindle assembly in human oocytes
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Juliane (Verified Customer) (10-25-2022)
Golgi and nucleus staining ok but cytoplasmic difficult