• Featured Product
  • KD/KO Validated

Eg5 Polyclonal antibody

Eg5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 23333-1-AP


EG5, HKSP, KIF11, kinesin family member 11, Kinesin like protein 1, Kinesin like protein KIF11, KNSL1, TR interacting protein 5, TRIP 5, TRIP5

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

23333-1-AP targets Eg5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Eg5 fusion protein Ag18695
Full Name kinesin family member 11
Calculated molecular weight 1056 aa, 119 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC136474
Gene symbol KIF11
Gene ID (NCBI) 3832
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.


Product Specific Protocols
WB protocol for Eg5 antibody 23333-1-APDownload protocol
IHC protocol for Eg5 antibody 23333-1-APDownload protocol
IF protocol for Eg5 antibody 23333-1-APDownload protocol
IP protocol for Eg5 antibody 23333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Oncol

Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway

Authors - Ting Xue

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu

Int J Biol Sci

KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer.

Authors - Dang Wei

Mol Carcinog

Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.

Authors - Benfang Wang
  • KD Validated

Am J Transl Res

Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma.

Authors - Xishan Li

Neurooncol Adv

KIF11 and KIF15 mitotic kinesins are potential therapeutic vulnerabilities for malignant peripheral nerve sheath tumors.

Authors - Ernest Terribas


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Iram (Verified Customer) (01-15-2021)

Eg5 antibody giving a very clean bands and good staining

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC and IF
  • Cell Tissue Type: Human colon cancer cells and tissue