Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
23333-1-AP targets Eg5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Eg5 fusion protein Ag18695|
|Full Name||kinesin family member 11|
|Calculated molecular weight||1056 aa, 119 kDa|
|Observed molecular weight||120-130 kDa|
|GenBank accession number||BC136474|
|Gene ID (NCBI)||3832|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.
|Product Specific Protocols|
|WB protocol for Eg5 antibody 23333-1-AP||Download protocol|
|IHC protocol for Eg5 antibody 23333-1-AP||Download protocol|
|IF protocol for Eg5 antibody 23333-1-AP||Download protocol|
|IP protocol for Eg5 antibody 23333-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Int J Oncol
Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway
Int J Cancer
Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.
Int J Biol Sci
KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer.
Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.
Am J Transl Res
Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma.
KIF11 and KIF15 mitotic kinesins are potential therapeutic vulnerabilities for malignant peripheral nerve sheath tumors.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iram (Verified Customer) (01-15-2021)
Eg5 antibody giving a very clean bands and good staining