VIRMA/KIAA1429 Polyclonal antibody

• Featured Product
• KD/KO Validated

VIRMA/KIAA1429 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 25712-1-AP

Synonyms

fSAP121, KIAA1429, MSTP054, Protein virilizer homolog, VIRMA, VIRMA/KIAA1429

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Validation Data Gallery

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  WB analysis of PC-3 using 25712-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25712-1-AP (KIAA1429 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 25712-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25712-1-AP (KIAA1429 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 25712-1-AP

  IP result of anti-VIRMA/KIAA1429 (IP:25712-1-AP, 4ug; Detection:25712-1-AP 1:600) with MCF-7 cells lysate 800 ug.

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  IHC staining of human breast cancer using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 25712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, SKOV-3 cells, MDA-MB-231 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

25712-1-AP targets VIRMA/KIAA1429 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VIRMA/KIAA1429 fusion protein Ag22449
• Full Name: KIAA1429
• Calculated Molecular Weight: 1812 aa, 202 kDa
• Observed Molecular Weight: 200-210 kDa
• GenBank Accession Number: BC113380
• Gene Symbol: KIAA1429
• Gene ID (NCBI): 25962
• RRID: AB_2880204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as oncogenic factor in breast cancer and liver cancer.

Publications

Species	Application	Title
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
human,mouse	WB,IHC,IP	Cell Mol Biol Lett; KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway; Authors - Xiaomin Chen ; KO Validated
human	WB	Aging (Albany NY); WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA.; Authors - Jiayi Wang
human	WB	Front Oncol; m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.; Authors - Congjun Zhang
human	WB,CoIP	Sci Rep; The m6A-methylase complex recruits TREX and regulates mRNA export.; Authors - Simon Lesbirel ; KD Validated
human	IHC	Med Sci Monit; Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.; Authors - Yingying Zhang