Tested Applications
| Positive WB detected in | HEK-293T cells, MDA-MB-231 cells, SKOV-3 cells, SW480 cells, PC-3 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SW480 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25712-1-AP targets VIRMA/KIAA1429 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22449 Product name: Recombinant human KIAA1429 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC113380 Sequence: MAVDSAMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDLFFNNVSKPSAPVFDRLGSLEYDENTSIIFRPNSKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQPQPSLKRNPKHADGEKEDQFNGSPPRPQPRGPRTPPGPPPPDDDEDDPVPLPVSGDKEEDAPHREDYFEPISPDRNSVPQEGQYSDEGEVEEEQQEEGEEDEDDVDVEEEEDEDEDDRRTVDSIPEEEEEDEEEEGEEDEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLLY Predict reactive species |
| Full Name | KIAA1429 |
| Calculated Molecular Weight | 1812 aa, 202 kDa |
| Observed Molecular Weight | 200-210 kDa |
| GenBank Accession Number | BC113380 |
| Gene Symbol | KIAA1429 |
| Gene ID (NCBI) | 25962 |
| RRID | AB_2880204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q69YN4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VIRMA/KIAA1429 antibody 25712-1-AP | Download protocol |
| IHC protocol for VIRMA/KIAA1429 antibody 25712-1-AP | Download protocol |
| IP protocol for VIRMA/KIAA1429 antibody 25712-1-AP | Download protocol |
| WB protocol for VIRMA/KIAA1429 antibody 25712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VIRMA/KIAA1429 Polyclonal antibody (Cat# 25712-1-AP) has 46 citations across journals including Nature Communications, Molecular Cell, Nucleic Acids Research, Journal of Experimental & Clinical Cancer Research, Science Advances, Cell Reports, Developmental Cell, and EMBO Reports. Research fields span m⁶A RNA modification, cancer biology (glioblastoma, colorectal, ovarian, hepatocellular, and other cancers), ferroptosis, immune regulation, neuroinflammation, and RNA processing.
| Species | Application | Title |
|---|---|---|
Nat Commun Epitranscriptomic regulation of mRNA stability in pivotal transcription factors modulates the second cell fate decision.
| ||
Mol Cell A lncRNA from the FTO locus acts as a suppressor of the m6A writer complex and p53 tumor suppression signaling | ||
Mol Cell EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma | ||
Nucleic Acids Res Armadillo domain of ARID1A directly interacts with DNA-PKcs to couple chromatin remodeling with nonhomologous end joining (NHEJ) pathway | ||
J Exp Clin Cancer Res CRISPR-Cas9 knockout screening identifies KIAA1429 as an essential gene in Ewing sarcoma | ||
J Exp Clin Cancer Res Phosphorylation of KIAA1429 promotes oxaliplatin resistance through activating the FZD7-Wnt signaling in BRAFV600E-mutated colorectal cancer |
Reviews
What customers say
The single review for 25712-1-AP is highly positive, rated 5 out of 5 stars. The reviewer reports using the antibody across multiple research projects and Western Blot, Immunohistochemistry, and Immunofluorescence applications at a 1:1000 dilution, describing it as working very well and expressing satisfaction with its performance. Overall, the feedback indicates reliable, consistent results across several techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (12-25-2025) | Our lab is enjoying this antibody, we used it for couple of our research projects, Glad they workout very well!
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