DYNC1H1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DYNC1H1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

54

Reviews (1)

Cat no : 12345-1-AP

Synonyms

DYNC1H 1

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Validation Data Gallery

• 

  WB analysis of HeLa using 12345-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1  antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12345-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1  antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12345-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1  antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12345-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1  antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12345-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1  antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12345-1-AP

  IP result of anti-DYNC1H1 (IP:12345-1-AP, 4ug; Detection:12345-1-AP 1:5000) with HeLa cells lysate 1085 ug.

• 

  IHC staining of mouse brain using 12345-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1  antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12345-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1  antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 12345-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12345-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12345-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1  antibody at dilution of 1:50.

• 

  IHC staining of human testis using 12345-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1  antibody at dilution of 1:50.

• 

  IHC staining of human breast cancer using 12345-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12345-1-AP (DYNC1H1  antibody at dilution of 1:50.

• 

  IF Staining of MCF-7 using 12345-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DYNC1H1 antibody (12345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of MCF-7 using 12345-1-AP

  Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody 12345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, mouse brain tissue, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue, human breast cancer tissue, human normal colon, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 32 publications below
• IHC: See 6 publications below
• IF: See 16 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

12345-1-AP targets DYNC1H1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYNC1H1 fusion protein Ag2999
• Full Name: dynein, cytoplasmic 1, heavy chain 1
• Calculated Molecular Weight: 4646 aa, 532 kDa
• Observed Molecular Weight: 532 kDa
• GenBank Accession Number: BC021297
• Gene Symbol: DYNC1H1
• Gene ID (NCBI): 1778
• RRID: AB_2261765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients..

Publications

Species	Application	Title
mouse	IF	Science; Mechanism of spindle pole organization and instability in human oocytes.; Authors - Chun So
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
human	WB	Nature; Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.; Authors - Henri-François Renard ; KD Validated
human	WB	Nat Cell Biol; Recycling of autophagosomal components from autolysosomes by the recycler complex.; Authors - Chuchu Zhou
human		Neuron; Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.; Authors - Andrew Kodani
human	WB	J Cell Biol; Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.; Authors - Hironori Inaba ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (08-24-2021)

Very good antibody, giving a clear and clean signal at the expected molecular weight.

• Applications: Western Blot

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