• Featured Product
  • KD/KO Validated

Rubicon Polyclonal antibody

Rubicon Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21444-1-AP


Baron, KIAA0226, RUBCN, Rubicon

Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman testis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A431 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21444-1-AP targets Rubicon in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rubicon fusion protein Ag13715
Full Name KIAA0226
Calculated Molecular Weight 972 aa, 109 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC033615
Gene Symbol KIAA0226
Gene ID (NCBI) 9711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rubicon (Run domain Beclin-1 interacting and cysteine-rich containing protein) consists of an N-terminal RUN domain, a middle region that includes its PI3K-binding domain (PIKBD), a coiled-coil domain (CCD), a serine-rich region (S-R), a helix-coil-rich domain (H-C) and a C-terminal Rubicon homology domain (RH) (PMID: 36288306). Rubicon is ubiquitously expressed in most tissue and organs.


Product Specific Protocols
WB protocol for Rubicon antibody 21444-1-APDownload protocol
IHC protocol for Rubicon antibody 21444-1-APDownload protocol
IF protocol for Rubicon antibody 21444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



ACS Nano

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia

Authors - Shishi Shen

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li


STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.

Authors - Cefan Zhou

Genes Dev

USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1.

Authors - Daichao Xu


RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Authors - Luis Muniz-Feliciano
  • KD Validated

Cell Mol Immunol

PMID: 28392573

Authors - Yushun Wan
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Daniel (Verified Customer) (07-21-2021)

The antibody seemed to work okay, although I had issues differentiating between autofluorescence and a positive signal. However, that is not the fault of the antibody, but rather my cells.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: iBMDMs