KI67 Recombinant antibody, PBS Only

KI67 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241499B1

Cat no : 84192-3-PBS

Synonyms

MKI67, Ki-67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67

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Validation Data Gallery

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  IHC staining of human tonsillitis using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of human thyroid cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of human thyroid cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

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Product Information

84192-3-PBS targets KI67 in IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: antigen identified by monoclonal antibody Ki-67
• Calculated Molecular Weight: 359 kDa
• GenBank Accession Number: NM_002417
• Gene Symbol: KI67
• Gene ID (NCBI): 4288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.