KCNMB4 Monoclonal antibody

KCNMB4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4H7G3

Cat no : 60122-1-Ig

Synonyms

BK channel subunit beta 4, BKbeta4, Hbeta4, K(VCA)beta 4, KCNMB4, Maxi K channel subunit beta 4, Slo beta 4

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Validation Data Gallery

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  WB analysis of fetal human brain using 60122-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 60122-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60122-1-Ig (KCNMB4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human brain tissue
• Positive IHC detected in: human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

60122-1-Ig targets KCNMB4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KCNMB4 fusion protein Ag5618
• Full Name: potassium large conductance calcium-activated channel, subfamily M, beta member 4
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC042446
• Gene Symbol: KCNMB4
• Gene ID (NCBI): 27345
• RRID: AB_2280832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Cytoprotective effects of the flavonoid quercetin by activating mitochondrial BKCa channels in endothelial cells.; Authors - Rafał Paweł Kampa
human	WB	Mitochondrion; Deficiency of the BKCa potassium channel displayed significant implications for the physiology of the human bronchial epithelium; Authors - Kamila Maliszewska-Olejniczak