KATNA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

KATNA1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 17560-1-AP

Synonyms

p60 katanin, Katanin p60 subunit A1, Katanin p60 ATPase-containing subunit A1, EC:5.6.1.1

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Validation Data Gallery

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  WB analysis using 17560-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17560-1-AP (KATNA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17560-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17560-1-AP (KATNA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17560-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17560-1-AP (KATNA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17560-1-AP

  IP result of anti-KATNA1 (IP:17560-1-AP, 4ug; Detection:17560-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human bowen disease using 17560-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17560-1-AP (KATNA1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HCT 116 cells, mouse brain tissue, rat brain tissue, HeLa cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 6 publications below

Product Information

17560-1-AP targets KATNA1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KATNA1 fusion protein Ag11753
• Full Name: katanin p60 (ATPase-containing) subunit A 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC050428
• Gene Symbol: KATNA1
• Gene ID (NCBI): 11104
• RRID: AB_10694670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Cell Res; Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.; Authors - Lei Wang
human	WB	Nat Cell Biol; Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex.; Authors - Kai Jiang
mouse	WB, IF	Neuron; Katanin p80 regulates human cortical development by limiting centriole and cilia number.; Authors - Wen F Hu
human	WB	Dev Cell; Microtubule Minus-End Stabilization by Polymerization-Driven CAMSAP Deposition.; Authors - Kai Jiang
mouse	IF	PLoS Biol; Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding.; Authors - Mary Mirvis
human,rat	IF	Elife; Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission; Authors - Dawn M Wenzel