TIP60/KAT5 Polyclonal antibody

• Featured Product
• KD/KO Validated

TIP60/KAT5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 10827-1-AP

Synonyms

60 kDa Tat interactive protein, cPLA(2) interacting protein, cPLA2, ESA1, Histone acetyltransferase KAT5, HIV 1 Tat interactive protein, HTATIP, HTATIP1, K(lysine) acetyltransferase 5, KAT5, Lysine acetyltransferase 5, PLIP, TIP, TIP60

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Validation Data Gallery

• 

  WB analysis of HepG2 using 10827-1-AP

  WB result of KAT5 antibody (10827-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAT5 transfected HepG2 cells.

• 

  WB analysis of PC-3 using 10827-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10827-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10827-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10827-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10827-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10827-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10827-1-AP (KAT5 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, rat liver tissue, HepG2 cells, Jurkat cells, HeLa cells, mouse liver tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Product Information

10827-1-AP targets TIP60/KAT5 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIP60/KAT5 fusion protein Ag1261
• Full Name: K(lysine) acetyltransferase 5
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 58 kDa, 70 kDa
• GenBank Accession Number: BC000166
• Gene Symbol: KAT5
• Gene ID (NCBI): 10524
• RRID: AB_2128431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis. KAT5 exists some isoforms with MV 53-60 kDa

Publications

Species	Application	Title
human	WB	Nature; ANP32E is a histone chaperone that removes H2A.Z from chromatin.; Authors - Arnaud Obri
mouse	WB	Nat Immunol; The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells.; Authors - Jing Geng
		Mol Cell; PMID: 28366643; Authors - Sophie Barral
human	WB	Nat Commun; Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress.; Authors - Zhiyuan You ; KD Validated
human	WB	Cancer Lett; Acetylation-dependent deubiquitinase USP26 stabilizes BAG3 to promote breast cancer progression; Authors - Jiazhou Liu
human	IF,IP	J Mol Cell Biol; Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis; Authors - Hameed Akbar

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