|Positive WB detected in||HEK-293T cells, Jurkat cells, human colon tissue, HeLa cells, HepG2 cells|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14951-1-AP targets KARS in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KARS fusion protein Ag6778|
|Full Name||lysyl-tRNA synthetase|
|Calculated molecular weight||68 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC004132|
|Gene ID (NCBI)||3735|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation.
Nucleic Acids Res
Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.
Nucleic Acids Res
Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.
Cell Chem Biol
Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.
Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.
Front Cell Infect Microbiol
Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kishor (Verified Customer) (01-30-2019)
This antibody working well against rat liver tissue and human cancer cells