KARS Polyclonal antibody

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  WB analysis using 14951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14951-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 14951-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human brown disease using 14951-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brown disease using 14951-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 14951-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KARS antibody (14951-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

KARS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14951-1-AP

Publications(11)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Lysyl-tRNA synthetase, Lysine--tRNA ligase, KIAA0070, KARS1, EC:2.7.7.-

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, human colon tissue, HeLa cells, HepG2 cells
• Positive IHC detected in: human brown disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 3 publications below

Product Information

14951-1-AP targets KARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KARS fusion protein Ag6778
• Full Name: lysyl-tRNA synthetase
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC004132
• Gene Symbol: KARS
• Gene ID (NCBI): 3735
• RRID: AB_2128158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15046
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KARS (lysyl-tRNA synthetase) is a gene that encodes an enzyme critical for protein synthesis, specifically responsible for attaching the amino acid lysine to its corresponding transfer RNA (tRNA) in both the cytoplasm and mitochondria. This enzyme plays a crucial role in the process of aminoacylation, which is essential for accurate protein translation from mRNA sequences. Mutations in the KARS gene have been associated with a variety of neurological and systemic disorders, reflecting the enzyme's importance in cellular function.

Publications

Species	Application	Title
human,mouse	WB	Sci Adv; Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation.; Authors - J Guerra
zebrafish	WB	Nucleic Acids Res; Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.; Authors - Qinghai Zhang
human	WB	Nucleic Acids Res; Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.; Authors - Haissi Cui
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	Hum Mutat; Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.; Authors - Benedetta Ruzzenente
human	IF	Front Cell Infect Microbiol; Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.; Authors - Tian Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kishor (Verified Customer) (01-30-2019)

This antibody working well against rat liver tissue and human cancer cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue

KARS Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:14951-1-AP)