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KARS Polyclonal antibody
KARS Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14951-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, Jurkat cells, human colon tissue, HeLa cells, HepG2 cells |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 9 publications below |
IF | See 2 publications below |
Product Information
14951-1-AP targets KARS in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | KARS fusion protein Ag6778 |
Full Name | lysyl-tRNA synthetase |
Calculated molecular weight | 68 kDa |
Observed molecular weight | 68 kDa |
GenBank accession number | BC004132 |
Gene symbol | KARS |
Gene ID (NCBI) | 3735 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for KARS antibody 14951-1-AP | Download protocol |
IHC protocol for KARS antibody 14951-1-AP | Download protocol |
IF protocol for KARS antibody 14951-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation. | ||
Nucleic Acids Res Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function. | ||
Nucleic Acids Res Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex. | ||
Cell Chem Biol Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer. | ||
Hum Mutat Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis. | ||
Front Cell Infect Microbiol Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kishor (Verified Customer) (01-30-2019) | This antibody working well against rat liver tissue and human cancer cells
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