KARS Polyclonal antibody

• Featured Product
• KD/KO Validated

KARS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 14951-1-AP

Synonyms

KARS, KARS2, KIAA0070, KRS, Lysine tRNA ligase, LysRS, lysyl tRNA synthetase

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Validation Data Gallery

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  WB analysis using 14951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14951-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 14951-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 14951-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14951-1-AP (KARS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14951-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14951-1-AP (KARS antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 14951-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KARS antibody (14951-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, human colon tissue, HeLa cells, HepG2 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Product Information

14951-1-AP targets KARS in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KARS fusion protein Ag6778
• Full Name: lysyl-tRNA synthetase
• Calculated molecular weight: 68 kDa
• Observed molecular weight: 68 kDa
• GenBank accession number: BC004132
• Gene symbol: KARS
• Gene ID (NCBI): 3735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Sci Adv; Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation.; Authors - J Guerra
zebrafish	WB	Nucleic Acids Res; Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.; Authors - Qinghai Zhang
human	WB	Nucleic Acids Res; Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.; Authors - Haissi Cui
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	Hum Mutat; Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.; Authors - Benedetta Ruzzenente
human	IF	Front Cell Infect Microbiol; Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.; Authors - Tian Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kishor (Verified Customer) (01-30-2019)

This antibody working well against rat liver tissue and human cancer cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue

KARS Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:14951-1-AP)