IL-2 Polyclonal antibody
IL-2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 26156-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PMA, Ionomycin and Brefeldin A treated EL-4 cells |
Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 5 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
Product Information
26156-1-AP targets IL-2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IL-2 fusion protein Ag24079 |
Full Name | interleukin 2 |
Calculated Molecular Weight | 19 kDa |
GenBank Accession Number | NM-008366 |
Gene Symbol | IL-2 |
Gene ID (NCBI) | 16183 |
RRID | AB_2880407 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
What is the calculated molecular weight of IL2?
19 kDa
Where is IL-2 produced?
IL-2 is primarily produced and secreted by CD4+ helper T cells in secondary lymphoid organs. CD8+ T cells, some B cells, and dendritic cells also produce IL-2.
What allows the protein to bind to its receptor?
The N-terminal 20 amino acids are essential for protein-receptor interaction.
What are the post-translational modifications of IL-2?
IL-2 may present glycosylation, yet the function of this modification has not been confirmed.
What is the role of IL-2?
IL-2 is a key immunoregulatory cytokine that promotes proliferation of helper T cells, upregulating its own secretion, while also increasing activity of regulatory T cells and providing negative feedback to its production and the immune response as a whole. IL-2 is also a growth factor for natural killer cells and an upregulator of B cell antibody expression and secretion.
What activates the IL-2 pathway?
Combined T cell antigen receptor and CD28 signals allow for maximum activation of the IL-2 pathway.
How does IL-2 expression change during an immune response?
During an immune response, CD4+ and CD8+ T cells will secrete increased amounts of IL-2 to be consumed by regulatory T cells.
How do the responses to strong and weak IL-2 signals differ?
Strong IL-2 levels promote antigen-specific T cells to differentiate into effector T cells, responding immediately to a stimulus or threat. Weak IL-2 signals, however, result in effector T cells to differentiate into helper or memory T cells.
What negatively regulates IL-2?
Immunoregulatory CD4+CD5+ T cells in inhibit IL-2 production by helper T cells.
(PMIDs: 22343569, 24376444, 15473953)
Protocols
Product Specific Protocols | |
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WB protocol for IL-2 antibody 26156-1-AP | Download protocol |
IHC protocol for IL-2 antibody 26156-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Invest New Drugs A novel tetravalent bispecific antibody targeting programmed death 1 and tyrosine-protein kinase Met for treatment of gastric cancer. | ||
J Cancer IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro. | ||
Peptides Synthesis of potent antagonists of receptors for growth hormone-releasing hormone with antitumor and anti-inflammatory activity. | ||
Lipids Health Dis Arachidonic acid induces macrophage cell cycle arrest through the JNK signaling pathway. | ||
Lung Anti-inflammatory Properties of the Alpha-Melanocyte-Stimulating Hormone in Models of Granulomatous Inflammation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lindsey (Verified Customer) (05-24-2021) | Great antibody--worked well from day 1!
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