IL-2 Polyclonal antibody

IL-2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26156-1-AP

Synonyms

Il 2, Il2, Il-2, interleukin 2



Tested Applications

Positive WB detected inPMA, Ionomycin and Brefeldin A treated EL-4 cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26156-1-AP targets IL-2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-2 fusion protein Ag24079
Full Name interleukin 2
Calculated Molecular Weight 19 kDa
GenBank Accession NumberNM-008366
Gene Symbol IL-2
Gene ID (NCBI) 16183
RRIDAB_2880407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the calculated molecular weight of IL2?

19 kDa

Where is IL-2 produced?

IL-2 is primarily produced and secreted by CD4+ helper T cells in secondary lymphoid organs. CD8+ T cells, some B cells, and dendritic cells also produce IL-2.

What allows the protein to bind to its receptor?

The N-terminal 20 amino acids are essential for protein-receptor interaction.

What are the post-translational modifications of IL-2?

IL-2 may present glycosylation, yet the function of this modification has not been confirmed. 

What is the role of IL-2?

IL-2 is a key immunoregulatory cytokine that promotes proliferation of helper T cells, upregulating its own secretion, while also increasing activity of regulatory T cells and providing negative feedback to its production and the immune response as a whole. IL-2 is also a growth factor for natural killer cells and an upregulator of B cell antibody expression and secretion. 

What activates the IL-2 pathway?

Combined T cell antigen receptor and CD28 signals allow for maximum activation of the IL-2 pathway. 

How does IL-2 expression change during an immune response?

During an immune response, CD4+ and CD8+ T cells will secrete increased amounts of IL-2 to be consumed by regulatory T cells. 

How do the responses to strong and weak IL-2 signals differ?

Strong IL-2 levels promote antigen-specific T cells to differentiate into effector T cells, responding immediately to a stimulus or threat. Weak IL-2 signals, however, result in effector T cells to differentiate into helper or memory T cells. 

What negatively regulates IL-2?

Immunoregulatory CD4+CD5+ T cells in inhibit IL-2 production by helper T cells.

 

(PMIDs: 22343569, 24376444, 15473953)

Protocols

Product Specific Protocols
WB protocol for IL-2 antibody 26156-1-APDownload protocol
IHC protocol for IL-2 antibody 26156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
human

Invest New Drugs

A novel tetravalent bispecific antibody targeting programmed death 1 and tyrosine-protein kinase Met for treatment of gastric cancer.

Authors - Weihua Hou
humanIHC

J Cancer

IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro.

Authors - Jia-Qian Hu
humanWB

Peptides

Synthesis of potent antagonists of receptors for growth hormone-releasing hormone with antitumor and anti-inflammatory activity.

Authors - Renzhi Cai
mouseWB

Lipids Health Dis

Arachidonic acid induces macrophage cell cycle arrest through the JNK signaling pathway.

Authors - Ziying Shen
humanWB

Lung

Anti-inflammatory Properties of the Alpha-Melanocyte-Stimulating Hormone in Models of Granulomatous Inflammation.

Authors - Abdolrazagh Hashemi Shahraki

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Lindsey (Verified Customer) (05-24-2021)

Great antibody--worked well from day 1!

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human kidney (FFPE); mouse lung (OCT/frozen)
IL-2 Antibody Immunofluorescence validation (1:100 dilution) in Human kidney (FFPE); mouse lung (OCT/frozen) (Cat no:26156-1-AP)