CD11B/Integrin Alpha M Polyclonal antibody

• Featured Product
• KD/KO Validated

CD11B/Integrin Alpha M Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 21851-1-AP

Synonyms

CD11b, CD11 antigen-like family member B, CR3A, CR-3 alpha chain, CR 3 alpha chain

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Validation Data Gallery

• 

  WB analysis using 21851-1-AP

  TF-1 cells were subjected to SDS PAGE followed by western blot with 21851-1-AP (CD11B/Integrin Alpha M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Insulinoma using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Insulinoma using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of THP-1 using 21851-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD11B/Integrin Alpha M antibody (21851-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: TF-1 cells
• Positive IHC detected in: human tonsillitis tissue, Insulinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 11 publications below
• IF: See 18 publications below
• FC: See 1 publications below

Product Information

21851-1-AP targets CD11B/Integrin Alpha M in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD11B/Integrin Alpha M fusion protein Ag16327
• Full Name: integrin, alpha M (complement component 3 receptor 3 subunit)
• Calculated Molecular Weight: 1152 aa, 127 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC096346
• Gene Symbol: CD11b
• Gene ID (NCBI): 3684
• RRID: AB_2878927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
human	IHC,WB	Aging Cell; Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts.; Authors - Zhi Chen
	FC	Biomaterials; Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.; Authors - Haoran Wang
	IF	ACS Appl Mater Interfaces; Catalysis-Mediated Male Contraception through Black Phosphorus Nanosheets; Authors - Lirong Yi
	IF	Int J Biol Macromol; Repairing gastric ulcer with hyaluronic acid/extracellular matrix composite through promoting M2-type polarization of macrophages; Authors - Renhao Ni
	IF	Biomater Sci; A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring; Authors - Ximeng Kang
human	WB	Int J Mol Sci; Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.; Authors - Krishnendu Khan