INTS4 Recombinant antibody

INTS4 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240579F7

Cat no : 83590-6-RR

Synonyms

MSTP093, MST093, Int4, 240579F7

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Validation Data Gallery

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  WB analysis using 83590-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83590-6-RR (INTS4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83590-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83590-6-RR (INTS4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 83590-6-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 83590-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 83590-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83590-6-RR against Human INTS4 were performed. The affinity constant is 0.149 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, mouse testis tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83590-6-RR is INTS4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: INTS4 fusion protein Ag9116
• Full Name: integrator complex subunit 4
• Calculated Molecular Weight: 963aa,108 kDa; 505aa,57 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC009995
• Gene Symbol: INTS4
• Gene ID (NCBI): 92105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).