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INTS4 Recombinant antibody, PBS Only

INTS4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240579F7

Cat no : 83590-6-PBS

Synonyms

MSTP093, MST093, Int4, 240579F7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INTS4 Recombinant antibody (83590-6-RR)

    WB analysis using 83590-6-RR (same clone as 83590-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83590-6-RR (INTS4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using INTS4 Recombinant antibody (83590-6-RR)

    IHC staining of human ovary cancer using 83590-6-RR (same clone as 83590-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83590-6-RR (INTS4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using INTS4 Recombinant antibody (83590-6-RR)

    IF Staining of U2OS using 83590-6-RR (same clone as 83590-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using INTS4 Recombinant antibody (83590-6-RR)

    IF Staining of HeLa using 83590-6-RR (same clone as 83590-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83590-6-RR

    Affinity and Kinetic Characterization of 83590-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83590-6-RR against Human INTS4 were performed. The affinity constant is 0.149 nM.

Product Information

The immunogen of 83590-6-PBS is INTS4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen INTS4 fusion protein Ag9116
Full Name integrator complex subunit 4
Calculated Molecular Weight963aa,108 kDa; 505aa,57 kDa
Observed Molecular Weight108 kDa
GenBank Accession NumberBC009995
Gene Symbol INTS4
Gene ID (NCBI) 92105
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).