INTS4 Polyclonal antibody

INTS4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16130-1-AP

Synonyms

INT4, integrator complex subunit 4, INTS4, MST093

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Validation Data Gallery

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  WB analysis using 16130-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16130-1-AP (INTS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16130-1-AP targets INTS4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INTS4 fusion protein Ag9116
• Full Name: integrator complex subunit 4
• Calculated Molecular Weight: 963aa,108 kDa; 505aa,57 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC009995
• Gene Symbol: INTS4
• Gene ID (NCBI): 92105
• RRID: AB_2296175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).