ING1-specific Polyclonal antibody

ING1-specific Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16185-1-AP

Synonyms

ING1, p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a

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Validation Data Gallery

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  WB analysis of human heart using 16185-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16185-1-AP (ING1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16185-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16185-1-AP (ING1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16185-1-AP

  IP result of anti-ING1-specific (IP:16185-1-AP, 2ug; Detection:16185-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: human heart tissue
• Positive IP detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16185-1-AP targets ING1-specific in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat'
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: inhibitor of growth family, member 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_198218
• Gene Symbol: ING1
• Gene ID (NCBI): 3621
• RRID: AB_2233908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING1 is the first identified one, interacting with TP53 and is a component of the p53 signaling pathway. Multiple alternatively spliced transcript variants encoding distinct isoforms of ING1 have been reported. This antibody recognizes all 24/33/47kd isoforms of ING1, but will not bind ING2, 3 or 4.