IMUP Polyclonal antibody

• Featured Product
• KD/KO Validated

IMUP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25561-1-AP

Synonyms

C19orf33, H2RSP, HAI 2 related small protein, IMUP, IMUP 1, IMUP 2

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Validation Data Gallery

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  WB analysis of A549 using 25561-1-AP

  WB result of IMUP antibody (25561-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMUP transfected A549 cells.

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  WB analysis using 25561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25561-1-AP (IMUP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 25561-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25561-1-AP (C19orf33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: EC109 cells, A549 cells, SGC-7901 cells, HeLa cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25561-1-AP targets IMUP in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IMUP fusion protein Ag22218
• Full Name: chromosome 19 open reading frame 33
• Calculated Molecular Weight: 106 aa, 11 kDa
• Observed Molecular Weight: 20 kDa, 22 kDa
• GenBank Accession Number: BC060319
• Gene Symbol: IMUP
• Gene ID (NCBI): 64073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). This polyclonal antibody raised against 1-106 aa of human IMUP can recognize both IMUP-1 and IMUP-2. The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).