IL-5RA Polyclonal antibody

IL-5RA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12655-1-AP

Synonyms

CD125, CDw125, HSIL5R3, IL 5 receptor subunit alpha, IL 5R alpha, IL 5R subunit alpha, IL 5RA, IL5R, IL5RA, IL-5RA, interleukin 5 receptor, alpha

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Validation Data Gallery

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  WB analysis of human peripheral blood platelets using 12655-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL-5RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12655-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL5RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human peripheral blood platelets, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12655-1-AP targets IL-5RA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-5RA fusion protein Ag3358
• Full Name: interleukin 5 receptor, alpha
• Calculated Molecular Weight: 48 kDa, 38 kDa
• Observed Molecular Weight: 70-75 kDa, 43 kDa
• GenBank Accession Number: BC027599
• Gene Symbol: IL-5RA
• Gene ID (NCBI): 3568
• RRID: AB_2127342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		J Allergy Clin Immunol; Mepolizumab does not alter levels of eosinophils, T cells, and mast cells in the duodenal mucosa in eosinophilic esophagitis; Authors - Sébastien Conus
human		Front Immunol; NET Formation in Bullous Pemphigoid Patients With Relapse Is Modulated by IL-17 and IL-23 Interplay.; Authors - Delphine Giusti