Rat IL-6 Recombinant antibody, PBS Only (Capture)

IL-6 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240003G2

Cat no : 83179-2-PBS

Synonyms

IL6, interleukin 6, ILg6, IL 6, Ifnb2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00264-2

  Sandwich ELISA standard curve of MP00264-2, Rat IL-6 Monoclonal Matched Antibody Pair - PBS only. 83179-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0800. 83179-3-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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  Sample test of MP00264-2

  Three rats were given an intraperitoneal injection of 100 μg/kg of LPS. All ratswere killed 4 hours after receiving LPS. Spleens were harvested and broken into individual cells by using homogenizer. Ratspleen conditioned media wascollected after culturing in 60 mL of RPMIsupplemented with 10% fetal bovine serum and stimulated with 1 μg/mL of LPS for 18 hours. An aliquot of the cell culture supernatant wasremoved, assayed for rat IL-6 and measured 6,449.6 pg/mL.

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  Sample test of MP00264-2

  One rat spleen was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant was removed, assayed for rat IL-6 and measured 179.7 pg/mL.

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  Sample test of MP00264-2

  Sixteen rat serum samples were evaluated for rat IL-6 in this assay. Fourteen samples were measured belowthe minimum standard of 62.5 pg/mL. The measured results of the two samples were 117.15 pg/mL and 133.51 pg/mL, respectively.

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  Cytometric bead array standard curve of MP00264-2

  Cytometric bead array standard curve ofMP00264-2, Rat IL-6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83179-2-PBS. Detection antibody: 83179-3-PBS. Standard: Eg0800. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00264-3

  Cytometric bead array standard curve ofMP00264-3, Rat IL-6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83179-2-PBS. Detection antibody: 83179-1-PBS. Standard: Eg0800. Range: 0.625-20 ng/mL.

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Product Information

83179-2-PBS targets IL-6 as part of a matched antibody pair:

MP00264-2: 83179-2-PBS capture and 83179-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00264-3: 83179-2-PBS capture and 83179-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 6
• Calculated Molecular Weight: 24 kDa
• GenBank Accession Number: NM_012589.2
• Gene Symbol: Il6
• Gene ID (NCBI): 24498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.