IL-2 Polyclonal antibody

IL-2 Polyclonal Antibody for ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

ELISA, FC (Intra)

Conjugate

Unconjugated

Cat no : 30199-1-AP

Synonyms

Il2, IL-2, interleukin 2

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Validation Data Gallery

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  FC experiment of rat splenocytes using 30199-1-AP

  1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ), and 0.5 ug CoraLite®594 Anti-Rat CD8a (OX-8) (CL594-65180, Clone: OX-8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of rat splenocytes using 30199-1-AP

  1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin treated rat splenocytes cells
• Positive FC detected in: PMA and ionomycin treated rat splenocytes cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30199-1-AP targets IL-2 in ELISA, FC (Intra) applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 2
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_053836
• Gene Symbol: Il2
• Gene ID (NCBI): 116562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.