IL-1alpha Recombinant antibody

IL-1alpha Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240431B5

Cat no : 83644-1-RR

Synonyms

IL1-alpha, Il1a, IL-1,IL1, IL1 alpha, Il 1a

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 83644-1-RR

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83644-1-RR (IL-1alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of RAW 264.7 using 83644-1-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS treated RAW 264.7 cells using IL-1alpha antibody (83644-1-RR, Clone: 240431B5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83644-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83644-1-RR against Mouse IL-1alpha were performed. The affinity constant is 1.15 nM.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IF/ICC detected in: LPS treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83644-1-RR is IL-1alpha Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 1 alpha
• Calculated Molecular Weight: 31kd
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_010554
• Gene Symbol: Il1a
• Gene ID (NCBI): 16175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.