IL-1R2 Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 60262-1-Ig (same clone as 60262-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60262-1-Ig (IL1R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60262-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60262-1-Ig (same clone as 60262-1-PBS)

  Immunofluorescent analysis of HeLa cells using 60262-1-Ig (IL1R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60262-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 60262-1-Ig (same clone as 60262-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IL-1R2 antibody (60262-1-Ig, Clone: 3H4H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60262-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

IL-1R2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 60262-1-PBS

Clone No.3H4H4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

IL1R2, 3H4H4, CD121 antigen-like family member B, CD121b, IL 1R 2

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Product Information

60262-1-PBS targets IL-1R2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4495

  Product name: Recombinant human IL-1R2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 24-347 aa of BC039031

  Sequence: AARSCRFRGRHYKREFRLEGEPVALRCPQVPYWLWASVSPRINLTWHKNDSARTVPGEEETRMWAQDGALWLLPALQEDSGTYVCTTRNASYCDKMSIELRVFENTDAFLPFISYPQILTLSTSGVLVCPDLSEFTRDKTDVKIQWYKDSLLLDKDNEKFLSVRGTTHLLVHDVALEDAGYYRCVLTFAHEGQQYNITRSIELRIKKKKEETIPVIISPLKTISASLGSRLTIPCKVFLGTGTPLTTMLWWTANDTHIESAYPGGRVTEGPRQEYSENNENYIEVPLIFDPVTREDLHMDFKCVVHNTLSFQTLRTTVKEASST

• Full Name: interleukin 1 receptor, type II
• Calculated Molecular Weight: 398 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC039031
• Gene Symbol: IL1R2
• Gene ID (NCBI): 7850
• RRID: AB_2881383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P27930
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.