IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit

VEGFA/VEGF Ready-to-use reagent kit for IHC.

Reactivity

Human

Sample Type

FFPE tissue

Cat no : KHC0735

Synonyms

VEGF, VEGFA, Vascular permeability factor, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A



Product Information

KHC0735 is a ready-to-use IHC kit for staining of VEGFA/VEGF. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Mouse Monoclonal
Secondary antibody type Polymer-HRP-Goat anti-Mouse
Reactivity Human

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms, some isoforms have homodimer forms. VEGF-A exists in at least seven homodimeric isoforms. Primary antibody in this kit can recognize all VEGFA isoforms.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms VEGF, VEGFA, Vascular permeability factor, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A

Publications

ApplicationTitle
IHC

Int J Biol Macromol

Examining the wound healing potential of curcumin-infused electrospun nanofibers from polyglutamic acid and gum arabic

Authors - Cuilin Cheng