IHCeasy SFRS2 Ready-To-Use IHC Kit

SFRS2 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse

Sample Type

FFPE tissue

Cat no : KHC0165

Synonyms

PR264, Protein PR264, SC 35, SC35, SFRS2, SFRS2A, Splicing component, 35 kDa, Splicing factor SC35, SRp30b, SRSF2



Product Information

KHC0165 is a ready-to-use IHC kit for staining of SFRS2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy SFRS2 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

SFRS2, also named as PR264 and Splicing component, 35 kDa, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. Some residue of SFRS2 is modified by phosphorylase and acetylase after translation. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. This kit does not cross react with SFRS2B.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms PR264, Protein PR264, SC 35, SC35, SFRS2, SFRS2A, Splicing component, 35 kDa, Splicing factor SC35, SRp30b, SRSF2