IHCeasy POLR2B Ready-To-Use IHC Kit
POLR2B Ready-to-use reagent kit for IHC.
Cat no : KHC1577
Synonyms
hRPB140, hsRPB2, POL2RB, POLR2B, POLR2B-Specific, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RPB2
Validation Data Gallery
Product Information
KHC1577 is a ready-to-use IHC kit for staining of POLR2B. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy POLR2B Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
POLR2B belongs to the RNA polymerase beta chain family. It is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is the second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. POLR2B proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. Primary antibody in this kit is specific to POLR2B.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | hRPB140, hsRPB2, POL2RB, POLR2B, POLR2B-Specific, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RPB2 |
