IHCeasy PDK1 Ready-To-Use IHC Kit
PDK1 Ready-to-use reagent kit for IHC.
Human, Mouse, Rat
Cat no : KHC0190
PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1
KHC0190 is a ready-to-use IHC kit for staining of PDK1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
|Product name||IHCeasy PDK1 Ready-To-Use IHC Kit|
|Sample type||FFPE tissue|
|Primary antibody type||Rabbit Polyclonal|
|Secondary antibody type||Polymer-HRP-Goat anti-Rabbit|
|Reactivity||Human, Mouse, Rat|
|Antigen Retrieval Buffer||100 mL||50×|
|Washing Buffer||100 mL ×2||20×|
|Blocking Buffer||5 mL||RTU|
|Primary Antibody||5 mL||RTU|
|Secondary Antibody||5 mL||RTU|
|Chromogen Component A||0.2 mL||RTU|
|Chromogen Component B||4 mL||RTU|
|Signal Enhancer||5 mL||RTU|
|Counter Staining Reagent||5 mL||RTU|
|Mounting Media||5 mL||RTU|
3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184).
|Storage Instructions||All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.|
|Synonyms||PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1|