IHCeasy Insulin Ready-To-Use IHC Kit
Insulin Ready-to-use reagent kit for IHC.
Cat no : KHC0004
Synonyms
ILPR, INS, Insulin, IRDN
Validation Data Gallery
Product Information
KHC0004 is a ready-to-use IHC kit for staining of insulin (INS). The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
Product name | IHCeasy Insulin Ready-To-Use IHC Kit |
Sample type | FFPE tissue |
Assay type | Immunohistochemistry |
Primary antibody type | Mouse Monoclonal |
Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
Reactivity | Human, Mouse, Rat |
Kit components
Component | Size | Concentration |
---|---|---|
Antigen Retrieval Buffer | 100 mL | 50× |
Washing Buffer | 100 mL ×2 | 20× |
Blocking Buffer | 5 mL | RTU |
Primary Antibody | 5 mL | RTU |
Secondary Antibody | 5 mL | RTU |
Chromogen Component A | 0.2 mL | RTU |
Chromogen Component B | 4 mL | RTU |
Signal Enhancer | 5 mL | RTU |
Counter Staining Reagent | 5 mL | RTU |
Mounting Media | 5 mL | RTU |
Datasheet | 1 Copy | |
Manual | 1 Copy |
Background Information
Insulin(INS) is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, pro-INS, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus.
Properties
Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
Synonyms | ILPR, INS, Insulin, IRDN |
Publications
Application | Title |
---|---|
J Physiol Biochem The effect of aspartame on accelerating caspase-dependent apoptosis of pancreatic islet via ZIPK/STAT3/caspase 3 signaling pathway |