IHCeasy GTF2F1 Ready-To-Use IHC Kit
GTF2F1 Ready-to-use reagent kit for IHC.
Human, Mouse, Rat
Cat no : KHC1583
BTF4, GTF2F1, RAP74, TF2F1, TFIIF, TFIIF alpha
KHC1583 is a ready-to-use IHC kit for staining of GTF2F1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
|IHCeasy GTF2F1 Ready-To-Use IHC Kit
|Primary antibody type
|Secondary antibody type
|Human, Mouse, Rat
|Antigen Retrieval Buffer
|100 mL ×2
|Chromogen Component A
|Chromogen Component B
|Counter Staining Reagent
In eukaryotic systems, the initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements, consisting of RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, B, D, E, F and H; and sequence specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, acting at an intermediate stage in initiation complex formation, binds directly to RNA polymerase II in solution and decrease the affinity of RNA polymerase II for nonspecific DNA. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.
|All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
|BTF4, GTF2F1, RAP74, TF2F1, TFIIF, TFIIF alpha