IHCeasy GLI3 Ready-To-Use IHC Kit

GLI3 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat no : KHC0137

Synonyms

ACLS, GCPS, GLI family zinc finger 3, GLI3, PAP A, PAPA, PAPA1, PAPB, PHS, PPDIV, Zinc finger protein GLI3



Product Information

KHC0137 is a ready-to-use IHC kit for staining of GLI3. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy GLI3 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms ACLS, GCPS, GLI family zinc finger 3, GLI3, PAP A, PAPA, PAPA1, PAPB, PHS, PPDIV, Zinc finger protein GLI3