Product Information
The PK10017 kit is designed for developing a complete IHC workflow using primary antibodies raised in rabbits. The kit contains all the basic reagents, starting from antigen retrieval buffer to mounting media, that would be required for IHC staining of tissue sections with rabbit primary antibodies. By simply optimizing the primary antibody concentration and a few operational parameters, a complete IHC workflow can be easily established using this kit.
Components
| Part NO. | Component | Size |
| HC001 | Antigen Retrieval Buffer (50× Tris-EDTA) | 100 mL |
| HC002 | Washing Buffer (30×) | 120 mL |
| HC003 | Blocking Buffer | 5 mL |
| HC000 | Quenching buffer (RTU) | 5 mL |
| HC004 | Primary Antibody Diluent (RTU) | 100 mL |
| HC005R | Polymer HRP-Goat anti-Rabbit Secondary Antibody | 5 mL |
| HC006 | Chromogen Component A | 0.2 mL |
| HC007 | Chromogen Component B | 4 mL |
| HC008 | Signal Enhancer | 5 mL |
| HC009 | Counter Staining Reagent | 5 mL |
| HC0010 | Mounting Media | 5 mL |
Size: 50-80 tests (may vary depending on tissue size)
Storage
All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Application Note
This kit is recommended for FFPE tissues and not for frozen sections.
This kit contains an antigen retrieval buffer based on Tris-EDTA by default, which in our experience is suitable for over 90% of targets. However, if a negative result is obtained using this buffer, we recommend trying alternative buffers listed below.
# PR30001 Sodium Citrate Antigen Retrieval Buffer 50x
# PR30014 Protease K Retrieval Buffer (A+B) (Heating is not required with this buffer)
Refer to user's manual for instructions on developing an IHC workflow using this kit.
Cited in Article as
PK10017, IHC Prep & Detect Kit for Rabbit Primary Antibody, Proteintech, IL, USA
Publications
| Application | Title |
|---|---|
Poult Sci Transcriptome identification of genes associated with uterus-vagina junction epithelial folds formation in chicken hens | |
Free Radic Biol Med Inhibition of ferroptosis by up-regulating Nrf2 delayed the progression of diabetic nephropathy | |
FASEB J The novel peptide PFAP1 promotes primordial follicle activation by binding to MCM5 | |
Int J Mol Sci A Diet Rich in Essential Amino Acids Inhibits the Growth of HCT116 Human Colon Cancer Cell In Vitro and In Vivo | |
Adv Sci (Weinh) Paternal Obesity-Induced H3K27me3 Elevation Leads to MANF-Mediated Transgenerational Metabolic Dysfunction in Female Offspring |
