IGFBP4 Polyclonal antibody

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  WB analysis using 30952-1-AP

  MDA-MB-231 cells lysates were subjected to SDS PAGE followed by western blot with 30952-1-AP (IGFBP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IGFBP4 Polyclonal Antibody for WB, ELISA

Cat No. 30952-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

BP 4, HT29 IGFBP, IBP 4, IBP4, IGF binding protein 4, IGFBP 4, IGFBP4

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30952-1-AP targets IGFBP4 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: insulin-like growth factor binding protein 4
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-40 kDa
• GenBank Accession Number: NM_001552.3
• Gene Symbol: IGFBP4
• Gene ID (NCBI): 3487
• RRID: AB_3669792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22692-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401).