IFIT5 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFIT5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 13378-1-AP

Synonyms

IFIT 5, IFIT5, RI58

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Validation Data Gallery

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  WB analysis of A375 using 13378-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13378-1-AP (IFIT5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13378-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13378-1-AP (IFIT5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 13378-1-AP

  IP result of anti-IFIT5 (IP:13378-1-AP, 4ug; Detection:13378-1-AP 1:300) with A375 cells lysate 2200ug.

• 

  IHC staining of human skin cancer using 13378-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13378-1-AP (IFIT5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 13378-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13378-1-AP (IFIT5 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: A375 cells, PC-3 cells
• Positive IP detected in: A375 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13378-1-AP targets IFIT5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFIT5 fusion protein Ag4202
• Full Name: interferon-induced protein with tetratricopeptide repeats 5
• Calculated Molecular Weight: 482 aa, 58 kDa
• Observed Molecular Weight: 56-58 kDa, 48 kDa
• GenBank Accession Number: BC025786
• Gene Symbol: IFIT5
• Gene ID (NCBI): 24138
• RRID: AB_10640300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.

Publications

Species	Application	Title
human	WB	J Immunol; IFIT5 Negatively Regulates the Type I IFN Pathway by Disrupting TBK1-IKKε-IRF3 Signalosome and Degrading IRF3 and IKKε.; Authors - Na Zhang
human	WB	Cell Signal; IFIT5 positively regulates NF-κB signaling through synergizing the recruitment of IκB kinase (IKK) to TGF-β-activated kinase 1 (TAK1).; Authors - Caishang Zheng ; KD Validated
Human	WB	Acta Biochim Biophys Sin (Shanghai); IFIT5 potentiates anti-viral response through enhancing innate immune signaling pathways.; Authors - Zhang Bianhong B
human	WB,IHC	Cell Death Dis; The roles and mechanism of IFIT5 in bladder cancer epithelial-mesenchymal transition and progression.; Authors - Jun Huang
human	IHC	Oncoimmunology; Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.; Authors - Chen Zhu
human	WB	mBio; PKR Binds Enterovirus IRESs, Displaces Host Translation Factors, and Impairs Viral Translation to Enable Innate Antiviral Signaling.; Authors - Mikhail I Dobrikov