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IDH3A Polyclonal antibody
IDH3A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 15909-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human skeletal muscle tissue, HepG2 cells, mouse brain tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 12 publications below |
IHC | See 3 publications below |
IF | See 4 publications below |
IP | See 1 publications below |
Product Information
15909-1-AP targets IDH3A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IDH3A fusion protein Ag8662 |
Full Name | isocitrate dehydrogenase 3 (NAD+) alpha |
Calculated molecular weight | 366 aa, 40 kDa |
Observed molecular weight | 40 kDa |
GenBank accession number | BC021967 |
Gene symbol | IDH3A |
Gene ID (NCBI) | 3419 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for IDH3A antibody 15909-1-AP | Download protocol |
IHC protocol for IDH3A antibody 15909-1-AP | Download protocol |
IF protocol for IDH3A antibody 15909-1-AP | Download protocol |
IP protocol for IDH3A antibody 15909-1-AP | Download protocol |
FC protocol for IDH3A antibody 15909-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. | ||
EMBO J Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues. | ||
J Invest Dermatol Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata. | ||
Cell Physiol Biochem Energy Metabolism in the Bone is Associated with Histomorphometric Changes in Rats with Hyperthyroidism. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Svitlana (Verified Customer) (03-19-2019) | SDS-PAGE: 15 ug protein lysate/well, 4-12% Bis-Tris.Transfer: Immobilon-FL transfer membranes O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1h at room temperature.Secondary Ab: IRDye 800CW Goat anti-Rabbit 1 h at room temperature.Lines on WB1. BioRad Precision Plus Protein standard2. Homogenate of human cortex tissue3. Non-synaptosomal mitochondria from human cortex4. Homogenate of mice brain tissue5. Non-synaptosomal mitochondria from mice brains
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