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ICMT Polyclonal antibody, PBS Only

ICMT Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17511-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICMT Polyclonal antibody (17511-1-AP)

    WB analysis using 17511-1-AP (same clone as 17511-1-PBS)

    Jurkat cells lysates were subjected to SDS PAGE followed by western blot with 17511-1-AP (ICMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17511-1-PBS in a different storage buffer formulation.

Product Information

17511-1-PBS targets ICMT in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name isoprenylcysteine carboxyl methyltransferase
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32-34 kDa
GenBank Accession NumberNM_012405
Gene Symbol ICMT
Gene ID (NCBI) 23463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Isoprenylcysteine carboxylmethyltransferase (ICMT), also known as PCCMT, is a multi-spanning endoplasmic reticulum integral membrane protein conserved among eukaryotic organisms. Methylation by ICMT is important for partitioning of CAAX proteins into lipid membranes as this modification neutralizes the negative charge on the carboxylate and increases the hydrophobicity of the modified protein (PMID: 25059662). Homozygous gene knockout of PPMT interferes with localization of K-Ras to the plasma membrane in mammalian cells and leads to the death of mouse embryos during gestation (PMID: 12141948).