Huntingtin Polyclonal antibody

Huntingtin Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 27206-1-AP

Synonyms

HTT, IT15, Huntington disease protein, Huntingtin, myristoylated N-terminal fragment, HD protein

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Validation Data Gallery

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  IHC staining of human cerebellum using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cerebellum using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 27206-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27206-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human brain using 27206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 27206-1-AP (Huntingtin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 27206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Huntingtin antibody (27206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human cerebellum tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human brain tissue, mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27206-1-AP targets Huntingtin in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Huntingtin fusion protein Ag25922
• Full Name: huntingtin
• Calculated Molecular Weight: 348 kDa
• GenBank Accession Number: NM_002111
• Gene Symbol: Huntingtin
• Gene ID (NCBI): 3064
• RRID: AB_2880799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTT(huntingtin), also named HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington's disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders, and dementia.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Florian (Verified Customer) (03-22-2024)

Primary antibody was tested for ICC, where no specific signal was observed. The antibody therefore was not able to specifically stain Huntingtin aggregates. We then also proceeded to detection of a WB, which resulted in a strong non-specific band. As the theoretical epitope of this antibody is of great interest for us, we would be very looking forward for improvements in this regard.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: HEK293T