Human TFF1 ELISA Kit
Cat no : KE00370
Synonyms
TFF1,Breast cancer estrogen-inducible protein,HP1.
Validation Data Gallery
Product Information
KE00370 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TFF1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TFF1. The assay recognizes human TFF1. An antibody specific for human TFF1 has been pre-coated onto the microwells. The human TFF1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TFF1 is added to detect the captured human TFF1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TFF1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7031 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| serum | 89% | 77%-99% |
| Urine | 99% | 92%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 965.3 | 54.8 | 5.7 |
| 2 | 20 | 204.4 | 7.5 | 3.7 |
| 3 | 20 | 46.8 | 3.9 | 8.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,012.1 | 58.0 | 5.7 |
| 2 | 24 | 243.5 | 13.0 | 5.3 |
| 3 | 24 | 57.6 | 2.5 | 4.4 |
Background Information
Trefoil factor 1 (TFF1) is also named as PNR-2, Polypeptide P1.A (hP1.A), Breast cancer estrogen-inducible protein (BCEI) and Protein pS2. TFF1 is one of three members of the trefoil factor family. While abundant TFF1 expression can be observed in the gastrointestinal epithelium, it is also expressed in other organs, such as the ileum, colon, and pancreas. The known function of TFF1 is to promote the regeneration of gastric mucosa induced by inflammatory injury by encouraging epithelial cell migration, and a similar regenerative effect is also found in the pancreas and liver. Importantly, recent studies have revealed that TFF1 also functions as a tumor suppressor to inhibit gastric, pancreatic, and hepatocellular carcinogenesis, presumably by inhibiting the Wnt/β-catenin pathway. In terms of the esophagus, TFF1 is not expressed in the squamous epithelium of the esophagus but is expressed in the submucosal gland and BE.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TFF1,Breast cancer estrogen-inducible protein,HP1. |