Human RBP4 ELISA Kit
Cat no : KE00270
Synonyms
Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4
Validation Data Gallery
Product Information
KE00270 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The RBP4 ELISA kit is to be used to detect and quantify protein levels of endogenous RBP4. The assay recognizes human RBP4. An antibody specific for RBP4 has been pre-coated onto the microwells. The RBP4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for RBP4 is added to detect the captured RBP4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RBP4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Saliva, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.083 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5950 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 88 | 74-99 |
| Cell culture supernatants | 82 | 72-92 |
| Urine | 89 | 73-104 |
| Saliva | 101 | 86-119 |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 12.3 | 0.65 | 5.3 |
| 2 | 20 | 18.4 | 0.75 | 4.0 |
| 3 | 20 | 40.8 | 0.92 | 2.3 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 12.6 | 0.25 | 2.0 |
| 2 | 24 | 18.0 | 0.60 | 3.3 |
| 3 | 24 | 37.9 | 1.68 | 4.4 |
Background Information
RBP4 (retinol-binding protein 4) belongs to the lipocalin family and is the specific carrier for retinol (Vitamin A) in the blood. It is mainly synthesized in the liver and circulates in the bloodstream bound to retinol in a complex with transthyretin. RBP4 acts as a drug target for ophthalmology research and reduction in serum RBP4 level inhibits lipofuscin without inhibiting the visual cycle. Urinary RBP4 could act as a marker for glomerular disease. And RBP4 also was identified as an adipokine that elevated in some insulin-resistant states. Measurement of serum RBP4 could be used to assess the risk of insulin resistance, type 2 diabetes, obesity, and cardiovascular disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4 |