Human Osteopontin/OPN ELISA Kit

Sensitivity

1.85 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat no : KE00233

Synonyms

BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin



Product Information

KE00233 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Osteopontin/OPN ELISA kit is to be used to detect and quantify protein levels of endogenous Osteopontin/OPN. The assay recognizes human Osteopontin/OPN. An antibody specific for Osteopontin/OPN has been pre-coated onto the microwells. The Osteopontin/OPN protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Osteopontin/OPN is added to detect the captured human Osteopontin/OPN protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human Osteopontin/OPN ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine, Human milk
Assay type Sandwich
Sensitivity 1.85 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 6696

Recovery

Sample TypeAverageRange
Human serum96%83%-117%
Human plasma94%77%-107%
Cell culture supernatants96%84%-117%
Urine92%77%-112%
Human milk93%86%-100%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201,000.930.33.0
220232.210.44.5
32054.52.34.2

InterAssay

Samplenmean ( pg/mL)SDCV%
124942.438.14.0
224214.711.85.5
32447.63.47.1

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues. OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Front Immunol

OPN promotes pro-inflammatory cytokine expression via ERK/JNK pathway and M1 macrophage polarization in Rosacea

Authors - Siyi Tang
humanserum,cell culture supernatants

Biochem Biophys Res Commun

Pirfenidone and nintedanib exert additive antifibrotic effects by the SPP1-AKT pathway in macrophages and fibroblasts

Authors - Chao Meng