Human NF-L ELISA Kit
Cat no : KE00305
Synonyms
68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, NF L, NF68, NFL
Validation Data Gallery
Product Information
KE00305 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NF-L ELISA kit is to be used to detect and quantify protein levels of endogenous human NF-L. The assay recognizes human NF-L. An antibody specific for human NF-L has been pre-coated onto the microwells. The human NF-L protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human NF-L is added to detect the captured human NF-L protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NF-L ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma，Cerebrospinal fluid |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4747 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 77%-109% |
| Human cerebrospinal fluid | 96% | 87%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 489.3 | 15.7 | 3.2 |
| 2 | 20 | 125.7 | 7.0 | 5.6 |
| 3 | 20 | 30.9 | 1.6 | 5.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 459.4 | 22.0 | 4.8 |
| 2 | 24 | 110.9 | 8.2 | 7.4 |
| 3 | 24 | 32.9 | 1.9 | 5.7 |
Background Information
NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 160 kDa NF-M and the 200 kDa NF-H to form neurofilaments. Neurofilaments are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homomeric 10-nm filaments. Accumulations of neurofilaments are a pathologic feature of several neurodegenerative diseases, including Amyotrophic Lateral Sclerosis (ALS), Alzheimer's disease, Parkinson's disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, NF L, NF68, NFL |