Human MMP-8 ELISA Kit

Sensitivity

4.6 pg/mL

Range

39-2500 pg/mL

Reactivity

Human

Cat no : KE00329

Synonyms

CLG1, HNC, Matrix metalloproteinase 8, MMP 8, MMP8, Neutrophil collagenase, PMNL CL, PMNL collagenase



Product Information

KE00329 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MMP-8 ELISA kit is to be used to detect and quantify protein levels of endogenous human MMP-8. The assay recognizes human MMP-8. An antibody specific for human MMP-8 has been pre-coated onto the microwells. The human MMP-8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MMP-8 is added to detect the captured human MMP-8 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human MMP-8 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant, Saliva,Tissue lysate
Assay type Sandwich
Sensitivity 4.6 pg/mL
Range 39-2500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 4317

Recovery

Sample TypeAverageRange
Human plasma81%76%-90%
Cell culture supernatant80%72%-85%
Saliva89%80%-106%
Tissue lysate89%80%-99%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201,249.2 37.6 3.0
220259.4 3.0 1.2
32075.8 1.4 1.8

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,276.1 46.5 3.6
224273.8 12.2 4.4
32470.3 2.7 3.9

Background Information

MMP-8 (Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells (PMID:15367487). The high-serum MMP-8 levels (>100 ng/mL) had poor cancer-specific survival (PMID: 29808017). MMP8 also has prognosis in patients with gastric cancer (PMID: 30192205).

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CLG1, HNC, Matrix metalloproteinase 8, MMP 8, MMP8, Neutrophil collagenase, PMNL CL, PMNL collagenase