Human IL-8 ELISA Kit

Sensitivity

1.0 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat no : KE00006

Synonyms

C X C motif chemokine 8, CXCL8, CXCL8,IL8, GCP1, IL 8, IL8, IL-8, interleukin 8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP 1, NAP1, Neutrophil activating factor, T cell chemotactic factor



Product Information

KE00006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL8 ELISA kit is to be used to detect and quantify protein levels of endogenous IL8. The assay recognizes human IL8. An antibody specific for IL8 has been pre-coated onto the microwells. The IL8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL8 is added to detect the captured IL8 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human IL-8 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.0 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3576

Recovery

Sample TypeAverageRange
Human plamsa100%80%-127%
Cell culture supernatants101%87%-114%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120127.35.34.1
220411.623.75.8
320804.444.75.6

InterAssay

Samplenmean ( pg/mL)SDCV%
124123.74.83.9
224420.917.14.1
324788.935.84.5

Background Information

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms C X C motif chemokine 8, CXCL8, CXCL8,IL8, GCP1, IL 8, IL8, IL-8, interleukin 8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP 1, NAP1, Neutrophil activating factor, T cell chemotactic factor

Publications

SpeciesSample TypeTitle
human

Cell Metab

Deamidation Shunts RelA from Mediating Inflammation to Aerobic Glycolysis.

Authors - Jun Zhao
humancell culture supernatants

Nat Commun

Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.

Authors - Michael Dietachmayr
humancell culture supernatants

Oncogene

IL-8-induced O-GlcNAc modification via GLUT3 and GFAT regulates cancer stem cell-like properties in colon and lung cancer cells.

Authors - Masahiro Shimizu
humancell culture supernatants

Oncogenesis

Erlotinib overcomes paclitaxel-resistant cancer stem cells by blocking the EGFR-CREB/GRβ-IL-6 axis in MUC1-positive cervical cancer.

Authors - Yaping Lv
humancell culture supernatants

Cell Death Dis

RNF183 promotes proliferation and metastasis of colorectal cancer cells via activation of NF-κB-IL-8 axis.

Authors - Rong Geng
humancell culture supernatants

Nanotoxicology

Role of inflammation in the malignant transformation of pleural mesothelial cells induced by multi-walled carbon nanotubes.

Authors - Xiaopei Huang