Human/Mouse HDAC1 ELISA Kit
Cat no : KE00390
Synonyms
Validation Data Gallery
Product Information
KE00390 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse HDAC1 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse HDAC1. The assay recognizes human/mouse HDAC1. An antibody specific for human/mouse HDAC1 has been pre-coated onto the microwells. The human/mouse HDAC1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse HDAC1 is added to detect the captured human/mouse HDAC1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse HDAC1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3065 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 74%-106% |
| Cell lysate | 90% | 78%-115% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 10.35 | 0.53 | 5.07 |
| 2 | 20 | 2.63 | 0.22 | 8.24 |
| 3 | 20 | 0.76 | 0.06 | 8.55 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 10.85 | 0.79 | 7.25 |
| 2 | 24 | 2.48 | 0.17 | 7.00 |
| 3 | 24 | 0.72 | 0.06 | 8.63 |
Background Information
Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/ acuc/ apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. HADC inhibition causes apoptosis in tumor cells and HDAC inhibitors may be developed as anti-cancer agents. This kit is used to quantify HDAC1 level in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms |