Human FN1 ELISA Kit
Cat no : KE00039
Synonyms
CIG, Cold insoluble globulin, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF
Validation Data Gallery
Product Information
KE00039 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FN1 ELISA kit is to be used to detect and quantify protein levels of endogenous FN1. The assay recognizes human FN1. An antibody specific for FN1 has been pre-coated onto the microwells. The FN1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for FN1 is added to detect the captured FN1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FN1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 47.2 pg/mL |
| Range | 125 - 8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2335 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 101% | 73%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 7,270.8 | 640.8 | 8.8 |
| 2 | 20 | 1,981.3 | 182.1 | 9.2 |
| 3 | 20 | 520.8 | 35.5 | 6.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 7,293.6 | 707.3 | 9.7 |
| 2 | 24 | 2,017.0 | 149.3 | 7.4 |
| 3 | 24 | 297.4 | 28 | 9.4 |
Background Information
Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CIG, Cold insoluble globulin, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF |