Human Clusterin ELISA Kit
Cat no : KE00312
Synonyms
AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2
Validation Data Gallery
Product Information
KE00312 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Clusterin ELISA kit is to be used to detect and quantify protein levels of endogenous human Clusterin. The assay recognizes human Clusterin. An antibody specific for human Clusterin has been pre-coated onto the microwells. The human Clusterin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human Clusterin is added to detect the captured human Clusterin protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Clusterin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.313 - 20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1191 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 101% | 87%-117% |
| Cell culture supernatant | 87% | 75%-105% |
| Urine | 87% | 81%-104% |
| Saliva | 96% | 87%-107% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 10.25 | 0.46 | 4.53 |
| 2 | 20 | 2.46 | 0.14 | 5.62 |
| 3 | 20 | 0.60 | 0.03 | 5.36 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 10.17 | 0.28 | 2.78 |
| 2 | 24 | 2.33 | 0.14 | 5.85 |
| 3 | 24 | 0.57 | 0.05 | 8.42 |
Background Information
Clusterin (also known as apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biological fluids. It is encoded by one single gene and cleaved posttranslationally into α and β subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, and regulating complement. Increased plasma clusterin levels have been associated with the severity, pathology, and progression of Alzheimer's disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2 |