Human CRP ELISA Kit

Sensitivity

0.02 ng/mL

Range

0.156-10 ng/mL

Reactivity

Human

Publications

4

Cat no : KE00004

Synonyms

C reactive protein, CRP, PTX1

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Validation Data Gallery

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  Standard curve of KE00004 Human CRP ELISA Kit.

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  Standard curve of KE00004 Human CRP ELISA Kit.

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Product Information

KE00004 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CRP ELISA kit is to be used to detect and quantify protein levels of endogenous CRP. The assay recognizes human CRP. An antibody specific for CRP has been pre-coated onto the microwells. The CRP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CRP is added to detect the captured CRP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

• Product name: Human CRP ELISA Kit
• Tests: 1 X 96 well plate
• Sample type: Serum, Plasma, Cell culture supernatants
• Assay type: Sandwich
• Sensitivity: 0.02 ng/mL
• Range: 0.156-10 ng/mL
• Reactivity: Human
• Tested applications: Sandwich ELISA
• Gene ID (NCBI): 1401

Recovery

Sample Type	Average	Range
Human plamsa	88%	76%-97%
Cell culture supernatants	115%	106%-123%

IntraAssay

Sample	n	mean ( ng/mL)	SD	CV%
1	20	2.05	0.16	7.6
2	20	3.15	0.28	8.8
3	20	7.45	0.68	9.2

InterAssay

Sample	n	mean ( ng/mL)	SD	CV%
1	24	1.93	0.13	6.9
2	24	2.81	0.26	9.2
3	24	4.97	0.48	9.6

Background Information

C-reactive protein (CRP) is an acute-phase serum protein predominately synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Properties

Storage Instructions	All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms	C reactive protein, CRP, PTX1

Publications

Species	Sample Type	Title
human		Int J Mol Sci; Interplay of Postprandial Triglyceride-Rich Lipoprotein Composition and Adipokines in Obese Adolescents; Authors - Silvia García-Rodríguez
human	serum	Exp Gerontol; Effect of intermittent hypoxic conditioning on inflammatory biomarkers in older adults.; Authors - Rafael Timon
human	serum	Dis Markers; Identification of Protein Expression Changes in Hepatocellular Carcinoma through iTRAQ.; Authors - Yuanyuan Zhang
human	serum	Ann N Y Acad Sci; Identification of maternal serum biomarkers for prenatal diagnosis of nonsyndromic orofacial clefts; Authors - Xinhuan Wang